abstract：:Human immunodeficiency virus (HIV) is the primary etiological agent of acquired immunodeficiency syndrome (AIDS). This virus can be transmitted by blood or blood products. Homosexual males (HM) are regarded as one of the high-risk groups for AIDS. Lymphocytes obtained from HM were used for cocultivation with fresh HIV...

journal_title：Journal of medical virology

authors： Lee YS,Baker L

更新日期：1987-12-01 00:00:00

abstract：:Human parvovirus B19 commonly infects children, causing erythema infectiosum (fifth disease). However, there is a significant adult population which has not been exposed to the virus and, consequently, does not have protective antibody. Recent reports have associated B19 infection during pregnancy with fetal death, al...

journal_title：Journal of medical virology

authors： Clewley JP,Cohen BJ,Field AM

更新日期：1987-12-01 00:00:00

abstract：:Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (greater than 6 months after illness) ...

journal_title：Journal of medical virology

authors： Burke DS,Nisalak A,Gentry MK

更新日期：1987-10-01 00:00:00

abstract：:To study the association between hepatitis B and schistosomiasis, 1,234 Egyptian males, ages 18 to 24, were interviewed, examined, and tested for Schistosoma mansoni infection and HBsAg. Sera from 91 (7.4%) of the study subjects were positive for HBsAg, and S. mansoni was found in the stools of 26.3%. There was no cor...

journal_title：Journal of medical virology

authors： Hyams KC,Mansour MM,Massoud A,Dunn MA

更新日期：1987-10-01 00:00:00

abstract：:In the present study the reactivity of IgG and IgM antibodies against HSV-1 structural and nonstructural proteins was analyzed by Western blot analysis (WBA) and radioimmunoprecipitation followed by polyacrylamide gel electrophoresis (RIPA-PAGE). It was demonstrated that IgM and IgG antibodies were directed against vi...

journal_title：Journal of medical virology

authors： Kühn JE,Dunkler G,Munk K,Braun RW

更新日期：1987-10-01 00:00:00

abstract：:Sera from 1,943 individuals from Auckland, New Zealand, were tested for the presence of serum antibodies to human T cell lymphotropic virus I (HTLV I), mainly with an enzyme-linked immunosorbent assay (ELISA) with cell extracts as target antigen. The individuals tested were blood donors and mostly Caucasian, but inclu...

journal_title：Journal of medical virology

authors： Reddy D,Okochi K,Woodfield DG,Judd W

更新日期：1987-08-01 00:00:00

abstract：:The effect of protamine, atropine, selenocystamine, taxifolin, and catechin on the infectivity and antigenicity of the cell culture-adapted hepatitis A virus (HAV) strain CF 53 was studied. The toxicity on uninfected PLC/PRF/5 cells was examined for each antiviral compound by morphological and biochemical methods, in ...

journal_title：Journal of medical virology

authors： Biziagos E,Crance JM,Passagot J,Deloince R

更新日期：1987-05-01 00:00:00

abstract：:The plaque reduction neutralization test (PRNT) has been used routinely in serological studies with such arenaviruses as Junin, Machupo, and Parana. However, difficulties have been encountered in using the PRNT for LCM virus, while conflicting views have been expressed about the reliability and efficacy of the test wi...

journal_title：Journal of medical virology

authors： Tomori O,Johnson KM,Kiley MP,Elliott LH

更新日期：1987-05-01 00:00:00

abstract：:Since outbreaks of severe acute hemorrhagic conjunctivitis occur worldwide [Hierholzer and Hatch, 1985] and the majority of the epidemics are caused by enterovirus 70 (EV-70), we developed an EV-70 IgM ELISA to simplify the diagnosis of these outbreaks. The test is based on the capture antibody technique and the use o...

journal_title：Journal of medical virology

authors： Wulff H,Anderson LJ,Pallansch MA,de Souza Carvalho RP

更新日期：1987-04-01 00:00:00

abstract：:Immunoglobulin class-specific determination by ELISA and a virus neutralization test (NT) were compared for the detection of the serum antibody responses after vaccination of infants with the RIT 4237 rotavirus vaccine (of bovine origin). A capture method was applied for specific IgM determination by ELISA because of ...

journal_title：Journal of medical virology

authors： Delem A,Vesikari T

更新日期：1987-03-01 00:00:00

abstract：:Thirty-two cerebrospinal fluid (CSF) samples from eighteen patients with confirmed herpes simplex encephalitis (HSE) were assayed by an indirect enzyme-linked immunosorbent assay (ELISA) for the presence of viral antigens. The results are expressed as an antigen ratio distinguishing between herpes simplex virus (HSV) ...

journal_title：Journal of medical virology

authors： Bos CA,Olding-Stenkvist E,Wilterdink JB,Scheffer AJ

更新日期：1987-02-01 00:00:00

abstract：:A sensitive microtitre radioimmunoassay was developed for detection of IgM antibodies to delta antigen. The assay was based on the selective binding of IgM from test sera to antihuman IgM (u-chain specific) fixed to wells of a microtitre plate, and utilized delta antigen extracted from the liver of an experimentally i...

journal_title：Journal of medical virology

authors： Dimitrakakis M,Waters MJ,Wootton A,Gust I

更新日期：1986-12-01 00:00:00

abstract：:Our studies of rotavirus challenge in adult volunteers enabled us to evaluate the relationship of pre-existing antirotavirus fecal IgA antibody to infection and illness and to investigate the local response to this infection. No relationship could be found between the pre-existing levels of fecal antirotavirus IgA ant...

journal_title：Journal of medical virology

authors： Bernstein DI,Ziegler JM,Ward RL

更新日期：1986-12-01 00:00:00

abstract：:In order to shed light on the mode of HTLV-I infection by mother-to-child transmission, we examined sera of school children in a highly endemic town on two separate occasions at a 6-year interval. The carrier rates in ages 15-17, 8.7 and 2.1%, were significantly higher than that in ages 6-8, 1.7 and 0.4%, in studies. ...

journal_title：Journal of medical virology

authors： Sugiyama H,Doi H,Yamaguchi K,Tsuji Y,Miyamoto T,Hino S

更新日期：1986-11-01 00:00:00

abstract：:Guinea pigs infected with Argentine hemorrhagic fever virus (Junin) were treated with pooled, homologous convalescent sera. Use of 15,000 or 5,000 therapeutic units of immune sera prevented all signs of illness when administered within 24 hr of infection. We could also prevent illness and death in infected guinea pigs...

journal_title：Journal of medical virology

authors： Kenyon RH,Green DE,Eddy GA,Peters CJ

更新日期：1986-11-01 00:00:00

abstract：:Within the framework of a comprehensive study of the correlation between enteric diseases and wastewater utilization in agricultural settlements (kibbutzim) the prevalence of several viral antibodies was examined among kibbutz residents and overseas volunteers. The latter were assumed to be a group highly susceptible ...

journal_title：Journal of medical virology

authors： Margalith M,Fattal B,Shuval HI,Morag A

更新日期：1986-10-01 00:00:00

abstract：:A comparative assessment of vidarabine and acyclovir in the treatment of varicella and disseminated zoster in immunosuppressed patients was undertaken. Thirty-eight immunosuppressed patients with varicella (N = 18) or disseminated zoster (N = 20) were treated intravenously with 10 mg/kg/day of vidarabine or 30 mg/kg/d...

journal_title：Journal of medical virology

authors： Vildé JL,Bricaire F,Leport C,Renaudie M,Brun-Vézinet F

更新日期：1986-10-01 00:00:00

abstract：:A hybrid cell line producing monoclonal antibodies recognizing an epitope encoded by the pre-(S)2 region of hepatitis B virus (HBV) genome was obtained by fusion of mouse myeloma cells with lymphocytes from mice immunized with HBV. The monoclonal antibody Mo-F124 secreted from the hybrid line reacted with the pre-S(2)...

journal_title：Journal of medical virology

authors： Budkowska A,Riottot MM,Dubreuil P,Lazizi Y,Brechot C,Sobczak E,Petit MA,Pillot J

更新日期：1986-10-01 00:00:00

abstract：:Inoue-Melnick virus (IMV) was stabilized to thermal inactivation at 50 degrees C by 1 M Na2SO4 but not by 1 M MgCl2, 1 M MgSO4, 2 M NaCl, and 2 M KCl. The latter salts enhanced the inactivation over that in distilled water. Na2SO4 stabilized IMV at 1 M concentration but enhanced inactivation at 0.1 M. Three types of I...

journal_title：Journal of medical virology

authors： Nishibe Y,Inoue YK,Melnick JL

更新日期：1986-10-01 00:00:00

abstract：:Only recently have we begun to fully realize the diversity of virions within a single human isolate of the group B Coxsackieviruses. These intratypic (strain) differences may be one of the important factors influencing pathogenesis, e.g., myocarditis vs. pancreatitis. Yet, until the virion strains within a type can be...

journal_title：Journal of medical virology

authors： Webb SR,Kearse KP,Foulke CL,Hartig PC,Prabhakar BS

更新日期：1986-09-01 00:00:00

abstract：:Alkali-dissociated, purified preparations of prototype coxsackievirus B4 release a protein kinase that catalyzes the incorporation of gamma-phosphate from 32P-labeled ATP into three virus capsid proteins (VP1, VP3, VP4), several additional proteins of the particle, and exogenous acceptor proteins. Using protamine sulf...

journal_title：Journal of medical virology

authors： Chatterjee NK,Nejman C

更新日期：1986-08-01 00:00:00

abstract：:In most developing countries, hepatitis B prevention is carried out early in life. In these countries, mobile immunization teams have a limited number of sessions to devote to each rural community; simultaneous administration of multiple antigens is thus normal practice. We compared the immune responses of Senegalese ...

journal_title：Journal of medical virology

authors： Yvonnet B,Coursaget P,Deubel V,Diop-Mar I,Digoutte JP,Chiron JP

更新日期：1986-08-01 00:00:00

abstract：:N-Acetylethyleneimine (AEI) was used to inactivate the avirulent Togavirus Semliki Forest virus (A774 strain) grown in chick embryo, Vero, and brain cell cultures. The purity of the virus preparation affected the kinetics of inactivation. The rate of inactivation increased with a rise in temperature from 5 to 40 degre...

journal_title：Journal of medical virology

authors： Amor S,Webb HE

更新日期：1986-08-01 00:00:00

abstract：:We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-li...

journal_title：Journal of medical virology

authors： Flaegstad T,Traavik T,Christie KE,Joergensen J

更新日期：1986-07-01 00:00:00

abstract：:Monoclonal antibodies were raised against hepatitis B core antigen (HBcAg) synthesized in Escherichia coli. They identified a single immunodominant determinant on both liver-derived and E. coli-derived HBcAg. Cross-inhibition studies demonstrated that HBsAg-containing human sera which contained antibodies to HBcAg (an...

journal_title：Journal of medical virology

authors： Ferns RB,Tedder RS

更新日期：1986-06-01 00:00:00

abstract：:The prevalence of coinfection and superinfection with the delta agent was studied in 2,645 hepatitis B surface antigen (HBsAg)-positive subjects from six countries and nine islands in the Western Pacific region. The study group comprised 262 patients with acute hepatitis B and 2,383 chronic carriers of HBsAg, of whom ...

journal_title：Journal of medical virology

authors： Dimitrakakis M,Crowe S,Gust I

更新日期：1986-04-01 00:00:00

abstract：:In nature, the cricetid Calomys musculinus is the principal host of Junin virus, the etiological agent of Argentine hemorrhagic fever. In the experimental infection, adult C. musculinus survived whereas newborns died after intraperitoneal inoculation with the XJ.Cl3 strain of Junin virus. The role of peritoneal macrop...

journal_title：Journal of medical virology

authors： Coulombié FC,Alché LE,Lampuri JS,Coto CE

更新日期：1986-03-01 00:00:00

abstract：:One hundred twenty strains of adenovirus types 1, 2, 5, and 6 (subgenus C), isolated at various times and places, were tested by neutralization and hemagglutination inhibition with two antisera of each type. Except for one type 5 strain they reacted largely type-specifically in both tests and neither qualitative nor q...

journal_title：Journal of medical virology

authors： Kaier B,Wigand R

更新日期：1986-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) test using polyvalent antigens and antisera was used to detect Coxsackie B virus-specific IgM responses in 329 patients admitted to the Coronary Care Unit, Wellington Hospital, New Zealand over a 12-month period. The sera of 30 of 153 (19.6%) patients with acute myocardial ...

journal_title：Journal of medical virology

authors： Lau RC

更新日期：1986-02-01 00:00:00

abstract：:To define better the human immune response to individual structural proteins of human cytomegalovirus (HCMV), 55 human sera with different IgG and IgM titres were studied for their reactivity with HCMV structural polypeptides separated by SDS-PAGE and electrotransferred to nitrocellulose paper. The results obtained sh...

journal_title：Journal of medical virology

authors： Landini MP,Re MC,Mirolo G,Baldassarri B,La Placa M

更新日期：1985-12-01 00:00:00

abstract：:Sera from 79 patients with acute self-limiting hepatitis, 17 patients with acute hepatitis B evolving into chronic HBsAg carriership, and 43 chronic HBsAg carriers without a history of acute hepatitis were analyzed for presence of hepatitis B virus (HBV)-DNA by a molecular hybridization technique. In acute self-limiti...

journal_title：Journal of medical virology

authors： Moestrup T,Hansson BG,Widell A,Blomberg J,Nordenfelt E

更新日期：1985-12-01 00:00:00

abstract：:Recently developed monoclonal antibodies against the 42,000-dalton major inner capsid protein were used in an enzyme immunoassay to subgroup a total of 156 rotavirus specimens obtained from Japanese infants and young children with acute gastroenteritis during the period between December 1981 and April 1983. Only 2 spe...

journal_title：Journal of medical virology

authors： Nakagomi O,Nakagomi T,Oyamada H,Suto T

更新日期：1985-09-01 00:00:00

abstract：:Immune-specific IFN (IFN) is produced by the peripheral blood mononuclear leukocytes (PBML) of greater than 95% of HSV-seropositive humans with infrequent recurrences of herpes labialis [Green, Yeh, and Overall, 1981]. However, herpes virus-induced immune-specific IFN was produced by PBML from only 33 of 48 (68.8%) pe...

journal_title：Journal of medical virology

authors： Green JA,Weiss PN,Yeh TJ,Spruance SL

更新日期：1985-07-01 00:00:00

abstract：:A one-year study of rotavirus prevalence was carried out using electron microscopy in Gabon, equatorial Africa. One hundred fifty-six nonhospitalized diarrheic children up to 10 years of age and 115 age-matched controls were investigated together with diarrheic adults and children over 10 years of age. Rotaviruses wer...

journal_title：Journal of medical virology

authors： Sitbon M,Lecerf A,Garin Y,Ivanoff B

更新日期：1985-06-01 00:00:00

abstract：:To determine whether unconventional pathogens causing subacute spongiform encephalopathy may be present in blood products, a newly developed hepatitis B vaccine and a widely used blood product were injected into mice and rats. As only a few aged mice in the test and the control groups showed spongiform encephalopathic...

journal_title：Journal of medical virology

authors： Tateishi J,Tsuji S

更新日期：1985-05-01 00:00:00

abstract：:To provide background for future hepatitis A vaccine trials, sera were collected from 0- to 4-year-old Liberian infants and their mothers on two occasions an average of 14.75 months apart and tested for antibody to hepatitis A virus (anti-HAV). The prevalence of anti-HAV rose from 2.5% in infants 0-6 months of age to ...

journal_title：Journal of medical virology

authors： Prince AM,Brotman B,Richardson L,White T,Pollock N,Riddle J

更新日期：1985-04-01 00:00:00

abstract：:Guinea pig infection with a highly passaged XJ prototype strain of Junín virus by the intramuscular route (IM) was carried out in order to study viral tropism modification in this host. The neurotropism of this strain was demonstrated by viral isolation, meningitis, and by the presence of Junín antigens as shown by im...

journal_title：Journal of medical virology

authors： Oubiña JR,Carballal G

更新日期：1985-02-01 00:00:00

abstract：:Faeces from 746 children less than 5 years old with acute gastroenteritis were screened for the presence of adenovirus particles or antigens by immunoelectron microscopy (IEM) and enzyme immunoassay (EIA). Thirty-five samples were positive by both IEM and EIA, two only by IEM, and two only by EIA, giving a total of 39...

journal_title：Journal of medical virology

authors： Leite JP,Pereira HG,Azeredo RS,Schatzmayr HG

更新日期：1985-02-01 00:00:00

abstract：:Twenty marmosets, male Callithrix jacchus, were used during this study. Fifteen of the marmosets were inoculated with 5,000 TCID50 of the attenuated XJC13 strain of Junin virus by intramuscular route and five were left as uninoculated controls. Animals were observed for a 420-day period. In order to carry out virologi...

journal_title：Journal of medical virology

authors： Avila MM,Frigerio MJ,Weber EL,Rondinone S,Samoilovich SR,Laguens RP,de Guerrero LB,Weissenbacher MC

更新日期：1985-01-01 00:00:00

abstract：:Intradermal inoculation of hepatitis B vaccine (HBsAg subtype adw) caused no side effects, but the vaccine was less immunogenic than following intramuscular administration. Intradermal inoculation does not, therefore, offer a major advantage to the generally used intramuscular immunization. A single multisite intrader...

journal_title：Journal of medical virology

authors： Zoulek G,Lorbeer B,Jilg W,Deinhardt F

更新日期：1984-01-01 00:00:00